Patrick Jacquemin
Group Leader
Transgenesis - TRSG
Younes Achouri
Institut de Duve
Avenue Hippocrate, 75 - B1.75.03
1200 Bruxelles
The Transgenesis core facility is managed by Dr Younes Achouri.
Logistic support to research
Provide transgene technology tools to research teams of UCLouvain to promote scientific excellence in biomedical research.
Provide access to transgene technology at cost price.
Open the access of the platform to research teams of other universities of the French-speaking community of Belgium, and collaborators of UCLouvain teams.
Share expertise
Share expertise in designing and creating transgenic mouse lines.
Attractiveness and Training
Reinforce the attractiveness of UCLouvain for young scientists willing to develop new research activities.
Offer training opportunities to PhD students and post-doctoral researchers.
Equipment
The platform comprises laboratory space, logistic space and housing in ventilated cages.
The equipment consists of:
- dissection microscope
- inverted microscope
- micromanipulators
- microinjection station
- incubators and flow hood
A room dedicated to cell cultures is made available in the de Duve Institute, in the laboratory of F. Lemaigre and P. Jacquemin.
This room is equipped with microscopes, laminar flow hood and incubator.
Offered service
CRISPR-Cas9 mouse model generation services
The Platform provides support for the design and generation of mouse models such as conditional knockout mice and targeted mutations, insertion of knock-in cassettes, using targeting vectors or single-stranded DNA donors. Projects and design are discussed with platform users. Our service includes:
- DNA donor design (single DNA donor or targeting vector)
- Guide RNAs design
- Ribonucleprotein complex (Cas9 and guide RNAs) and DNA donor injection into fertilized oocytes
- Transfer of injected oocytes into oviduct of pseudo-pregnant female mice
- Platform users take charge of ordering the guide RNAs and single strand DNA donor (Ultramer or Megamer).
Generation of transgenic mice by injection of DNA construct in fertilized oocytes
The platform also provides help in the design of the DNA constructs and shares available plasmids.
Homologous recombination
Platform users take charge of generating DNA constructs. Culture, electroporation and selection of ES cells is performed by platform users under supervision of Dr Y. Achouri. The platform takes charge of injecting the recombined ES cells in blastocysts and reimplanting the injected blastocysts into oviduct of pseudo-pregnant female mice.
Team
Animal caretaker
Core Facility Manager
Variations in the poly-histidine repeat motif of HOXA1 contribute to bicuspid aortic valve in mouse and zebrafish.
Odelin G, Faucherre A, Marchese D, Pinard A, Jaouadi H, Le Scouarnec S; FranceGenRef Consortium; Chiarelli R, Achouri Y, Faure E, Herbane M, Théron A, Avierinos JF, Jopling C, Collod-Béroud G, Rezsohazy R, Zaffran S.
Nat Commun (2023) 14(1):1543.
Mesp1 controls the chromatin and enhancer landscapes essential for spatiotemporal patterning of early cardiovascular progenitors.
Lin X, Swedlund B, Ton MN, Ghazanfar S, Guibentif C, Paulissen C, Baudelet E, Plaindoux E, Achouri Y, Calonne E, Dubois C, Mansfield W, Zaffran S, Marioni JC, Fuks F, Göttgens B, Lescroart F, Blanpain C.
Nat Cell Biol (2022) 24(7):1114-1128
Dynamic Regulation of Expression of KRAS and Its Effectors Determines the Ability to Initiate Tumorigenesis in Pancreatic Acinar Cells.
Assi M, Achouri Y, Loriot A, Dauguet N, Dahou H, Baldan J, Libert M, Fain JS, Guerra C, Bouwens L, Barbacid M, Lemaigre FP, Jacquemin P.
Cancer Res (2021) 81(10):2679-2689.
EOMES interacts with RUNX3 and BRG1 to promote innate memory cell formation through epigenetic reprogramming.
Istaces N, Splittgerber M, Lima Silva V, Nguyen M, Thomas S, Le A, Achouri Y, Calonne E, Defrance M, Fuks F, Goriely S, Azouz A.
Nat Commun (2019) 10(1):3306
Avenue Hippocrate 75 - 1200 Brussels - BELGIUM
VAT : BE0423 837 837
Tel : +32 2 764 75 50