Didier Vertommen
Senior Investigator & MASSPROT Core Facility Manager
Mass Spectrometry - MASSPROT
Didier Vertommen
Institut de Duve
Avenue Hippocrate, 74 - B1.74.02
1200 Bruxelles
MASSPROT core facility, managed by Dr Didier Vertommen, provides proteomics services principally by liquid chromatography coupled to mass spectrometry. Our tools are ESI HR/AM Orbitrap coupled to nanoLC. An HDX-MS platform is also available. We specialize in the identification and quantification of proteins from complex samples. The platform also has unique expertise in the identification and quantification of post-translational modifications, which can be a critical asset in the fields of new antibiotic discovery, diabetes, cancer and immunotherapy
Equipment
- Orbitrap Fusion Lumos Tribrid Mass spectrometer: HR/AM, < 1 ppm mass accuracy, resolving power up to 500.000 FWHM, multiple fragmentation methods: CID, HCD, ETD, EThcD
- Orbitrap Exploris 240: HR/AM, <1 ppm mass accuracy, resolving power up to 240.000 FWHM, HCD fragmentation
- Two Thermo Scientific Ultimate 3000 RSLC nano/cap UHPL Systems, for peptide separation prior to MS analysis
- One Thermo Scientific Vanquish Neo nano/cap UHPL system
- One HDX LEAP robot from Trajan to perform automated HDX-MS experiments
- Two Agilent HPLC systems for off-line fractionation of complex peptide mixtures
Offered service
The facility provides a full range of proteomics services allowing the isolation, identification and quantitation of proteins of interest for researchers within UCLouvain and to external academic and industrial clients. Many types of analysis are possible: from measurements of masses of intact proteins to differential quantitative phosphoproteomics workflows. The MS platform is always happy to provide advice on experimental design and sample preparation and strongly encourages researchers to discuss their proteomics needs at the very early stages of their research with members of the MASSPROT platform to allow tailored experimental design.
Please contact Dr Didier Vertommen (Proteomics Facility Manager at DDUV) to discuss the proteomic approaches available for your project and the cost of the various services.
Research
Projects
- Nucleox (FWO-FNRS): Nuclear Oxidative Stress Signaling: decoding ROS signals during stress
- HYGIEIA (Fondation Roi Baudouin): understanding and addressing the SARS-CoV2 pandemic with systems biology and network medicine
Diseases
Team
Academic supervisor
Research assistant
Specific post-translational modifications of soluble tau protein distinguishes Alzheimer’s disease and primary tauopathies
Kyalu Ngoie Zola N, Balty C, Pyr Dit Ruys S, Vanparys AAT, Huyghe NDG, Herinckx G, Johanns M, Boyer E, Kienlen-Campard P, Rider MH, Vertommen D, Hanseeuw BJ
Nat Commun (2023) 14(1):3706
Oncogenic CALR mutant C-terminus mediates dual binding to the thrombopoietin receptor triggering complex dimerization and activation
Papadopoulos N, Nédélec A, Derenne A, Şulea TA, Pecquet C, Chachoua I, Vertenoeil G, Tilmant T, Petrescu A-J, Mazzucchelli G, Iorga BI, Vertommen D, Constantinescu SN
Nat Commun (2023) 14(1):1881
A draft map of the human ovarian proteome for tissue engineering and clinical application
Ouni E, Vertommen D, Chiti MC, Dolmans M-M, Amorim CA
Mol Cell Proteomics (2019) 18(Suppl 1):S159-S173
Repairing oxidized proteins in the bacterial envelope using respiratory chain electrons
Gennaris A, Ezraty B, Henry C, Agrebi R, Vergnes A, Oheix E, Bos J, Leverrier P, Espinosa L, Szewczyk J, Vertommen D, Iranzo O, Collet JF, Barras F
Nature (2015) 528(7582):409-412
Avenue Hippocrate 75 - 1200 Brussels - BELGIUM
VAT : BE0423 837 837
Tel : +32 2 764 75 50